Product Name | (2,3-Dioleoyloxy-propyl)-trimethylammonium -chloride (For Injection),DOTAP |
Chemical Name | N-[1-(2,3-DIOLEYLOXY)PROPYL]-N,N,N-TRIMETHYLAMMONIUM |
CAS No. | 132172-61-3 |
NMPA CDE registration no. | / |
USFDA DMF No. | / |
Quality grade | Injection |
Quality standards | Manufacturer's standard |
Molecular Formula | C42H80ClNO4 |
Applications and examples | The polar headgroup of the cationic lipid DOTAP can stably bind to negatively charged DNA molecules through electrostatic interactions, forming a stable complex that enables effective gene transfection. This transfection reagent is very gentle, avoiding the cytotoxicity associated with other transfection methods, and has higher transfection efficiency compared to other lipid-based transfection reagents. Therefore, DOTAP can be used as a commercial transfection reagent. |
Product code | O02002 |
Package sizes | 1g, 10g, 50g |
Storage condition | This product should be transported and stored in a sealed and light-protected container at a temperature of −20 ± 5°C. It should be kept away from strong oxidizing agents. Under proper use and storage conditions, it is valid for three years, with retesting conducted annually. |
Kecekapan transfection tinggi: sebagai reagen transfection gen dalam produk komersial, DOTAP menawarkan proses transfection yang lebih lembut. Berbanding dengan ejen transfection berasaskan lipid lain, ia membantu mengurangkan kesan sitotoksik dan memastikan kecekapan transfection yang lebih tinggi.
Penghantaran yang disasarkan: DOTAP boleh berfungsi sebagai komponen tambahan dalam organ terpilih yang mensasarkan tiny lipid (jenis) (LNPs). Menggabungkan 10%-15% DOTAP di LNPs memudahkan penghantaran ubat yang disasarkan kepada limpa, sambil meningkatkan kandungan DOTAP hingga 50% mencapai kecekapan penghantaran paru-paru yang optimum.
DOTAP adalah biologi lengai, terbiodegradasi, tidak toksik, dan immunogenic. Kepalanya kutub stably mengikat kepada molekul DNA negatif dikenakan melalui interaksi elektrostatik, membentuk kompleks stabil secara spontan yang memasuki sel-sel dan menyampaikan asid nukleik ke dalam sitoplasma tanpa menyebabkan kerosakan kepada organisma. Oleh itu, ia sering digunakan sebagai vektor untuk reagen eukariot transfection dan vaksin DNA.
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